R & D

Amazing Biotech Pvt Ltd is research-oriented service lab started in Marakanam. It located in Chennai coast surrounded by both farm and hatchery. We used to get samples from hatchery and farmers. We developed our research platform from the knowledge accrued from farmers. We are using our strength to help the farmers, and the effect of ABPL is to train young mind to get opportunity in agriculture, aquaculture field.

To reach across length and breadth of India, we developed service-based lab in Marakanam, Ongole and Kakinada. We also gave consultancy services to several labs located in Kakinada, Ongole, Eluru, Amalapuram, Nellore, Thirupoondi, and a water quality lab at Thondi.

Our R & D developed several products to help farmers, also developed indigenous diagnosis kit for pathogen detection. We have our collaboration with several research labs to understand and address several issues in sustainable aqua, agriculture. Currently, we are running three projects with our collaborator. Our collaborators are:

Our research group is leaded by qualified scientists who can guide young people to address the problems. We are handling both agriculture and aquaculture related problems with our knowledge in microbiology and biotechnology. We plan to develop small instruments with our collaborators to test pond parameters, disease occurrence in their location itself to take quick action. We are also organizing several training programs, project works for the university, college students.

S.NO	Product	Name & Address of the Technical Collaborator	History of Collaboration
1	a) Enumeration of functional based bacteria and its potential use in aqua industry b) Climate change and its adaptation of seagrass and its associated communities	Dr. Amit Kumar Scientist Centre for Climate Change Studies Sathyabama University Jeppiaar Nagar Rajiv Gandhi Salai Sholinganallur, Chennai 600119 Tamil Nadu	a)As we published few papers and a thesis from our last collaboration project. We started our second project in that similar line, in which, we are aim to find functional based bacteria and the potential use in aqua industry. Progress: Enumerated few potential bacteria and done preliminary work Our collaboration is to enumerate the potential sea grass associated bacteria from the site and its potential use b) In this project represent the future sea in the climate change context, in which we are looking into the associated bacterial communities. Finding out the beneficial bacteria for the production of bioactive compound for aquaculture. Functional studies and its potential use in industry
2	Pond water Vibrio correlation and quorum sensing	Dr Grasian Immanuel Manonmaniam Sundaranar University, Rajakamangalam Tirunelveli, Tamil Nadu	In this research association, for the detection of Vibrio, maintenance of the strain, correlation studies etc. Performance trials are in progress. Progress: Few trails made. Data produced Papers published The data has been used and corelated to industrial shrimp hatchery production Dr. Balasubramaniam, Amazing biotech Pvt ltd is contributing as a member in board of studies in Animal Science Department, Manonmaniam sundaranar university, Tirunelveli Dr. Sasi Kumar, Amazing biotech Pvt ltd is contributing as a member in board of studies in Biotechnology Department, Manonmaniam Sundaranar university, Tirunelveli
3	a) qRT- PCR kit development for Shrimp and Fish diseases.Vibrio parahaemolyticusand Vibrio harveryii detection by qRT-PCR method. b) Carbon capturing and Utilization model in biofloc shrimp culture b) The prevalence vibrio and its correlation with M3 -PL syndrome is studied qRT-PCR kit for the bacterial species identification is developed for V. alginolyticus and V. vulnificus	Dr. Joseph Selvin Department of Microbiology Pondicherry University	Amazing Biotech is already collaborating for the past 2 years on this project work. We already developed detection kit for Vibrio parahaemolyticus and Vibrio harveyii. Now, we are studying Vibrio alginolyticus and Vibrio vulnificus and its harmful effect in aquaculture production. Progress: Detection kit developed and validated in the aqua field. Kit have been developed with candidate gene approach. Trail and validation done. Dr.Vinu S S, Amazing biotech Pvt ltd is contributing as a member in board of studies in Microbiology Department, Pondicherry university We got results and going to publish the causative agents for the major M3-PL problem in shrimp hatcheries We are trying in-field kit development using gene editing technology along with Dr. Shegal Kiran
4	a) Enumeration of seaweed associated bacteria and its potential use in aqua industry – PHA/PHB production & Lipopeptide production and use in aquaculture b) Extraction of active compound from red algae	Dr. Shegal Kiran Department of food technology Pondicherry University	“A PROCESS OF PREPARATION OF ENHANCED PROBIOTIC POTENTIAL EXHIBITING SHRIMP FEED FORMULATION” patented – No - 558129 Two bacteria isolated and its potential use has been published by Dr. Shegal kiran and his team. Most of the on-field trials has been supported by Amazing Biotech Pvt Ltd. The pilot product development has been done in our R&D premises. Two patent application process going on – 1) “A Process of Extraction of antibacterial activity exhibiting Fucosterol from Red Alga Gracilaria textorii” 2) “A vibriocidal formulation for prevention/control of vibriosis in shrimp aquaculture”

Patents, articles, and publications

Patents

Granted Patent:
Title: A process of preparation of enhancing probiotic potential exhibiting shrimp feed formulation
Patent No: 558129
Date of Grant: 17 January 2025

Patents Under Preparation:

A process of extraction of antibacterial activity exhibiting fucosterol from red alga Gracilaria textori
A vibriocidal formulation for prevention/control of vibriosis in shrimp aquaculture

Articles

Siva, V., & Kumar, A. (2024). Vibrio becomes lethal to aquaculture. Aquafocus, Vol. 2, Issues 3 & 4, July 2024.
Siva, V., Talukder, A., & Kumar, A. (2023). Bio pigments and their applications in aquaculture industry. Aquafocus, Vol. 1, Issue 3, April 2023.
Siva, V., Balasubramaniam, V., & Ashok Kumar, P. A. (2021). Digital PCR – The way forward in aquaculture disease detection. Aquaculture Spectrum, Vol. 4, Issue 11, November 2021.

Publications

John, J., Siva, V., Kumari, R., Arya, A., & Kumar, A. (2020). Unveiling cultivable and uncultivable halophilic bacteria inhabiting Marakkanam saltpan, India and their potential for biotechnological applications. Geomicrobiology Journal, 37(8), 691–701.
John, J., Siva, V., & Kumar, A. (2020). Isolation and characterization of saltpan bacteria for developing bacterial consortium for the complete removal of nitrogen pollution from aquaculture pond. In Conference Proceedings An Insight on Environmental Toxicology (ISBN: 978-93-5396-157-2).
John, J., Siva, V., Richa, K., Arya, A., & Kumar, A. (2019). Life in high salt concentrations with changing environmental conditions: Insights from genomic and phenotypic analysis of Salinivibrio sp. Microorganisms, 7(11), 577.
John, J., Siva, V. S., Kumar, A., Ebenezer, V., Raika, P., Khalifa, U., ... & Sanjeevi, P. (2017). Physiological tolerance of the early life history stages of freshwater prawn (Macrobrachium rosenbergii De Man, 1879) to environmental stress. bioRxiv, 159244.